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tradd  (Cell Signaling Technology Inc)


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    Structured Review

    Cell Signaling Technology Inc tradd
    Tradd, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 93/100, based on 15 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+tradd/TRADD+Rabbit+mAb/pm41413112-228-68-69
    Average 93 stars, based on 15 article reviews
    tradd - by Bioz Stars, 2026-09
    93/100 stars

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    Related Articles

    other:

    Article Title: HAF Prevents Hepatocyte Apoptosis and Hepatocellular Carcinoma through Transcriptional Regulation of the NF-κB pathway
    Article Snippet: Primary rabbit polyclonal antibodies for HAF was as described , whereas antibodies against TRADD, RIP1, NEMO, pp65, p65, p50, IkBa, caspase-3 and -8, pRIP1, pRIP3, RIP3 were purchased from Cell Signaling Technology (Danvers, MA), antibodies against pp50 and NEMO were from Santa Cruz Biotechnology (Dallas, TX), and Bcl-xS antibody was from ThermoFisher Scientific (Waltham, MA).

    Article Title: Shigella type-III secretion system effectors counteract the induction of host inflammation and cell death
    Article Snippet: Anti-TRADD , Cell Signaling Technology , #3694.

    Western Blot:

    Article Title: EFHD2 suppresses intestinal inflammation by blocking intestinal epithelial cell TNFR1 internalization and cell death
    Article Snippet: .. For immunoblot analysis, the following antibodies were used: anti-caspase-7 (1:1000, CST, #9492), anti-caspase-3 (1:1000, CST, #9662), anti-caspase-8 (1:2000, CST, #9746), anti-cleaved-caspase-8 (1:1000, CST, #9496, #8592), anti-p-MLKL (1:1000, CST, #91689), anti-MLKL (1:1000, CST, #14993, #37705), anti-p-RIPK1 (1:1000, CST, #65746, #31122), anti-RIPK1 (1:1000, CST, #3493), anti-p-IKKα/β (1:2000, CST, #2697), anti-IKKα (1:1000, CST, #2682), anti-IKKβ (1:1000, CST, #8943), anti-p-P65 (1:2000, CST, #3033), anti-P65 (1:1000, CST, #8242), anti-TRADD (1:1000, CST, #3684), anti-cIAP1 (1:1000, CST, #7065), anti-cIAP2 (1:1000, CST, #3130), anti-TRAF2 (1:1000, CST, #4724), anti-Rab5 (1:1000, CST, #3547), anti-EEA1 (1:1000, CST, #3288), anti-p-Cofilin (1:1000, CST, #3313), and anti-Cofilin (1:1000, CST, #5175), anti-Na/K-ATPase (1:2000, Santa Cruz, sc-48345), anti-β-actin (1:5000, MBL, M177-3), anti-EFHD2 (1:1000, Genetex, GTX108080), anti-Flag (1:5000, Sigma Aldrich, F1804), anti-TNFR1 (1:600, Proteintech, 21574-1-AP), anti-cFLIP (1:1000, CST, #56343), anti-Bcl-xl (1:1000, CST, #2764), and anti-Bcl-2 (1:1000, CST, #3498). .. Anti-FLAG M2 Magnetic Beads (Sigma Aldrich) and RIPK1 (1:100, CST, #3493) were used for immunoprecipitation assays in HCT-116 cells.

    Incubation:

    Article Title: Deciphering the Mechanisms Underlying the Antitumor Effects of Eucalyptus Essential Oil and Its Component 3-Cyclohexene-1-Methanol Against Human Colon Cancer Cells
    Article Snippet: .. Thereafter, membranes were incubated overnight with indicated primary antibodies (anti-p53, anti-pp53 (Ser15), anti-p27, anti-p21, anti-Cyclin D1, anti-CDK2, anti-CDK4, anti-phospho CDC2 (Tyr15), anti-phospho Rb, anti-TRADD, anti-Caspase 8, anti-Caspase 7, anti-Caspase 9, anti-AIF, anti-Caspase 3, anti-Bad, anti-phospho Bad (Ser112), anti-Cytochrome C, anti-Bik, anti-Bax, anti-Puma, anti-AKT, anti-phospho AKT (Ser473), anti-phospho ERK1/2 (Thr202/Tyr204), anti-ERK1/2, anti-phospho p38 (Thr180/Tyr182), anti-p38, anti-phospho SAPK/JNK (Thr183/Tyr185), anti-HSP90, anti-β-actin) from cell Signaling Technology (Danvers, MA, USA) at a dilution of 1:1000. β-actin and HSP90 were used as the loading control. .. Immunoreactivity was detected by using horseradish peroxidase-conjugated anti-rabbit or anti-mouse IgG (Promega, Madison, WI, USA) and visualized in an X-ray film or using a ChemiDoc MP Imaging System (Bio-Rad, Hercules, CA, USA) after incubation with enhanced Chemiluminescent (ECL, Pierce, Rockford, IL, USA).

    Control:

    Article Title: Deciphering the Mechanisms Underlying the Antitumor Effects of Eucalyptus Essential Oil and Its Component 3-Cyclohexene-1-Methanol Against Human Colon Cancer Cells
    Article Snippet: .. Thereafter, membranes were incubated overnight with indicated primary antibodies (anti-p53, anti-pp53 (Ser15), anti-p27, anti-p21, anti-Cyclin D1, anti-CDK2, anti-CDK4, anti-phospho CDC2 (Tyr15), anti-phospho Rb, anti-TRADD, anti-Caspase 8, anti-Caspase 7, anti-Caspase 9, anti-AIF, anti-Caspase 3, anti-Bad, anti-phospho Bad (Ser112), anti-Cytochrome C, anti-Bik, anti-Bax, anti-Puma, anti-AKT, anti-phospho AKT (Ser473), anti-phospho ERK1/2 (Thr202/Tyr204), anti-ERK1/2, anti-phospho p38 (Thr180/Tyr182), anti-p38, anti-phospho SAPK/JNK (Thr183/Tyr185), anti-HSP90, anti-β-actin) from cell Signaling Technology (Danvers, MA, USA) at a dilution of 1:1000. β-actin and HSP90 were used as the loading control. .. Immunoreactivity was detected by using horseradish peroxidase-conjugated anti-rabbit or anti-mouse IgG (Promega, Madison, WI, USA) and visualized in an X-ray film or using a ChemiDoc MP Imaging System (Bio-Rad, Hercules, CA, USA) after incubation with enhanced Chemiluminescent (ECL, Pierce, Rockford, IL, USA).



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